Shedding of the indicated APMV strains from the upper respiratory tract of rhesus macaques.^*

*Rhesus macaques in groups of four were inoculated simultaneously by the intranasal and intratracheal routes with a 1-ml inoculum per site containing 6.0 log10 PFU per ml of the indicated virus on day 0. Nasal washes were performed daily from day 0 to day 10 and on day 12. †The 50 percent egg infectious dose (EID50) virus titer (expressed as log10 EID50 per mL), determined as described in Materials and Methods. Limit of detection was 0.8 log10 per mL. For calculation of daily means, a value of 0.4 was used for samples with no detectable virus. ‡For calculation of daily means, a value of 0.4 log10 per mL was used for samples with no detectable virus. §Shedding of wt APMV-5 was measured by limiting dilution assay of nasal wash fluid on Vero cells, followed by virus detection using a neuraminidase assay.* 四只一组的恒河猴（Rhesus macaques）于第0天通过鼻内与气管内途径同时接种，每个接种部位使用1 mL接种液，其中每毫升含6.0 log₁₀ 蚀斑形成单位（PFU）的指定病毒。自第0天至第10天每日进行鼻腔灌洗，并于第12天额外开展一次鼻腔灌洗。 † 50%鸡胚感染量（EID50, 50 percent egg infectious dose）病毒滴度以log₁₀ EID50/mL表示，测定方法详见材料与方法。检测限为0.8 log₁₀/mL。计算每日平均值时，未检出病毒的样本赋值为0.4。 ‡ 计算每日平均值时，未检出病毒的样本赋值为0.4 log₁₀/mL。 § 野生型APMV-5（wt APMV-5）的排毒情况通过以下方式测定：对鼻腔灌洗液进行有限稀释试验（limiting dilution assay）并接种Vero细胞，随后采用神经氨酸酶试验（neuraminidase assay）检测病毒。