Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection [smallRNA-Seq]

Rice blast is a recurrent fungal disease, and resistance to fungal infection is a complex trait. Therefore, a comprehensive examination of rice transcriptome and its variation during fungal infection is necessary to understand the complex gene regulatory networks. In this study, adopting Next-Generation Sequencing we profiled the transcriptomes and microRNAomes of rice varieties, one susceptible and the other resistant to M. oryzae, at multiple time points during the fungal infection. In order to investigate variations of rice transcriptome and microRNAome in response to blast infection, we sequenced, profiled, compared and analyzed the gene expressions of two rice cultivars, one susceptible (CO39) and the other resistant (C101LAC) to M. oryzae, at different time points after M. oryzae infection (Fig. 1).

稻瘟病是一种周期性暴发的真菌病害，水稻对真菌侵染的抗性属于复杂性状。因此，为阐明真菌侵染过程中水稻复杂的基因调控网络，全面解析水稻转录组（transcriptome）及其在侵染过程中的动态变化至关重要。本研究采用下一代测序（Next-Generation Sequencing）技术，对感病与抗病两种水稻品种在真菌侵染进程中的多个时间点，分别完成了转录组与微小RNA组（microRNAome）的组学表征分析。为探究水稻转录组和微小RNA组在响应稻瘟病菌（M. oryzae）侵染时的表达变异，本研究进一步对两个水稻品种——感病品种CO39与抗病品种C101LAC——在稻瘟病菌侵染后的不同时间点的基因表达情况开展了测序、比较与解析工作（图1）。