Validation results for sgRNAs that were highlighted in the pooled CRISPR screens and selected for further characterization.

For each SNP, we characterized the sgRNA with the best overall performance in the CRISPR screens. For each sgRNA, we combined results from 3–4 biological replicates. Within each replicate, we compared the overall distributions of the endothelial cell readouts (between SNP sgRNA and negative control sgRNA) using the Kolmogorov-Smirnov test (Fig 3B). To combine results across replicates and assess significance, we used a simple one-tailed Student’s t-test. MFI, mean fluorescence intensity from the flow cytometer.
(XLSX)

针对每一个单核苷酸多态性（Single Nucleotide Polymorphism, SNP），我们对在CRISPR筛选中整体表现最优的单向导RNA（single-guide RNA, sgRNA）进行了表征。针对每一条sgRNA，我们整合了3~4次生物学重复的实验结果。在每一次重复实验中，我们采用柯尔莫哥洛夫-斯米尔诺夫检验（Kolmogorov-Smirnov test）对比了SNP靶向sgRNA与阴性对照sgRNA之间内皮细胞检测指标的整体分布情况（图3B）。为整合各重复实验的结果并评估统计学显著性，我们采用了单尾学生t检验。MFI指流式细胞仪测得的平均荧光强度（mean fluorescence intensity）。
(XLSX)