[E-MTAB-434] Transcription profiling by high throughput sequencing of zebra fish tissues and developmental stages

Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages using the Illumina Genome Analyzer. Paired-end sequence data has been generated using polyA selected RNA from a range of zebrafish tissues and developmental stages using the Illumina Genome Analyzer. These data have been used to improve the gene annotation of the zebrafish genome. Study description: Zebrafish total RNA was extracted from embryonic and adult tissue, then polyA selected. After fragmentation and reverse transcription Illumina sequencing libraries were prepared. Paired-end sequence runs were performed with 36, 37, 54 and 76 base reads on the Illumina Genome Analyzer.本研究采用Illumina基因组分析仪（Illumina Genome Analyzer），对涵盖多种组织与发育阶段的斑马鱼转录组开展测序。研究以取自多种斑马鱼组织及发育阶段的polyA富集RNA为材料，生成了双端测序数据，测序平台为Illumina基因组分析仪。上述数据已被用于优化斑马鱼基因组的基因注释工作。研究详情：从斑马鱼胚胎与成体组织中提取总RNA，随后进行polyA富集；经片段化与反转录后构建Illumina测序文库，再使用Illumina基因组分析仪完成读长为36、37、54及76碱基的双端测序。