Regulatory consequences of TARBP2 knock-down in293T cell-line

siRNA-mediated knock-down followed by transcriptomic profiling revealed that silencing of TARBP2 resulted in a significant up-regulation of sRSE-carrying transcripts. Two independent siRNAs were used to knock down TARBP2 in metastatic 293T cells. The cells were then subjected to transcripome profiling in comparison to mock-transfected cells.通过小干扰RNA（siRNA）介导的基因敲低联合转录组谱分析，研究发现敲低TARBP2基因可显著上调携带sRSE的转录本水平。本研究使用两种独立的siRNA，在转移性293T细胞中敲低TARBP2基因，随后对处理后的细胞开展转录组谱分析，并以模拟转染的细胞作为对照进行对比。