Expression data changes induced by 3D conditions in T-ALL cells [CCRF-CEM cells]. Expression data changes induced by 3D conditions in T-ALL cells [CCRF-CEM cells]

3D constrained conditions promoted H3K4 methylation in ALL cells that altered the global chromatin configuration and transcriptional changes related to cell cycle and DNA replication. Overall design: CCRF-CEM cells (T-ALL cell line) were used for RNA extraction and hybridization on Affymetrix microarrays. CCRF-CEM cells were cultured in suspension or 3D conditions (a type I collagen matrix of 1,7 mg/mL) for 1 hour. Cells were lised and mRNA was extracted to compare expression profiles. We used 2 replicates of each sample.

三维约束培养条件可促进急性淋巴细胞白血病（Acute Lymphoblastic Leukemia, ALL）细胞中的H3K4甲基化，进而改变细胞全局染色质构象并引发与细胞周期、DNA复制相关的转录组改变。实验设计：本研究采用CCRF-CEM细胞（T-ALL细胞系）进行RNA提取，并在Affymetrix微阵列芯片上完成杂交反应。将CCRF-CEM细胞分别置于悬浮培养体系与三维培养体系（1.7 mg/mL的I型胶原基质）中培养1小时，收集细胞并裂解后提取mRNA，以对比两组的基因表达谱。每个样本设置2个生物学重复。