Single-cell RNA sequencing of human salivary gland derived mesenchymal stromal cells under cytokine treatment conditions

Mesenchymal stromal cells (MSCs) reside in tissue stroma and are associated with potent immunomodulatory and trophic properties. Furthermore, the immunomodulatory properties of MSCs are traditionally optimized with cytokine stimulation such as with IFNγ or both IFNγ and TNFα. The effect of these cytokines on MSC(SG) are unknown. We report the study of labial salivary gland-derived MSCs (MSC[SG]) as a potential MSC source with beneficial immunomodulatory and trophic properties. We studied MSC(SG) after cytokine pre-licensing with vehicle,  IFNγ, TNFα, or both IFNγ and TNFα (dual treatment). , Mesenchymal stromal cells were isolated from three biologically distinct human labial salivary glands.Patients were healthy controls who had head and neck cancer that did not involve the labial salivary gland and did not have any dryness symptoms. After expansion to 80% confluence, each of the three biologiacal replicates were treated with vehicle,  60 ng/mL of IFNγ and 10 ng/mL of TNFα for 24 hours. , , # Single-cell RNA sequencing of human salivary gland derived mesenchymal stromal cells under cytokine treatment conditions

Dataset DOI: [10.5061/dryad.9ghx3ffv4](10.5061/dryad.9ghx3ffv4)

## Description of the data and file structure

Single-cell RNA sequencing of salivary gland-derived mesenchymal stromal cells treated with cytokines

#### Methods

Labial salivary gland-derived mesenchymal stromal cells from human participants. The cells were isolated, expanded, and treated with the following conditions: 1) vehicle; 2) 60 ng/mL IFN-gamma; 3) 10 ng/mL TNF-alpha; 4) both 60 ng/mL IFN-gamma and 10 ng/mL TNF-alpha. Treatment occurred for 24 hours before cryopreservation.

#### Description of the data and file structure

We include fastq files for nucleotide sequence information along with quality scores for each sequenced nucleotide in forward and reverse for each treatment condition and each subject.

We include DEGs in several comparisons. markers_humanmsc_all includes a table with the ...,

间充质基质细胞（mesenchymal stromal cells, MSCs）定居于组织基质中，具备强效的免疫调节与营养支持特性。传统上，通常通过干扰素γ（IFNγ）或同时联合干扰素γ与肿瘤坏死因子α（TNFα）等细胞因子刺激，以优化MSCs的免疫调节性能。不过，此类细胞因子对唾液腺来源的MSCs（MSC[SG]）的作用尚不明确。本研究聚焦于唇腺来源的MSCs（MSC[SG]），将其作为一种具备潜在有益免疫调节与营养支持特性的MSCs来源进行探究。我们对经载体对照、干扰素γ、肿瘤坏死因子α，或同时联合干扰素γ与肿瘤坏死因子α（双因子处理）进行细胞因子预致敏后的唇腺MSCs展开了研究。

本研究从3例生物学背景各异的人体唇腺中分离得到间充质基质细胞。受试者为健康对照者，均患有未累及唇腺且无任何口干症状的头颈部恶性肿瘤。将细胞扩增至汇合度80%后，对3份生物学重复样本分别采用载体对照、60 ng/mL干扰素γ以及10 ng/mL肿瘤坏死因子α处理24小时。

# 细胞因子处理条件下人唾液腺来源间充质基质细胞的单细胞RNA测序
数据集DOI: [10.5061/dryad.9ghx3ffv4](10.5061/dryad.9ghx3ffv4)

## 数据与文件结构说明
经细胞因子处理的唾液腺来源间充质基质细胞的单细胞RNA测序

#### 研究方法
本研究从人类受试者的唇腺中分离得到间充质基质细胞。对细胞进行分离、扩增，并采用以下4种处理条件：1）载体对照；2）60 ng/mL 干扰素γ；3）10 ng/mL 肿瘤坏死因子α；4）同时添加60 ng/mL 干扰素γ与10 ng/mL 肿瘤坏死因子α。细胞经上述处理24小时后进行冷冻保存。

#### 数据与文件结构详情
本数据集为每名受试者、每种处理条件提供了正向与反向测序的核苷酸序列信息fastq文件，以及每个测序核苷酸对应的质量评分文件。
本数据集还包含多组比较分析得到的差异表达基因（differentially expressed genes, DEGs）数据：markers_humanmsc_all 包含一张涵盖……的表格，