Cell autonomous roles for AP-2alpha in lens vesicle separation and maintenance of the lens epithelial cell phenotype.. Mus musculus

This is a comparative microarray analysis of LE-AP-2a mutants vs. wild-type P0 littermate lenses. This analysis revealed differential gene expression of 415 mRNAs, including reduced expression of genes important for maintaining lens epithelial cell phenotype, such as E-cadherin. Keywords: Differential gene expression Overall design: Biological triplicates of mouse lenses were analyzed (three wild-type lens samples and three Le-AP-2 mutant samples)

本研究针对LE-AP-2a突变体与野生型P0同窝仔鼠晶状体开展比较微阵列分析（comparative microarray analysis）。本次分析共鉴定出415个差异表达mRNA，其中包含维持晶状体上皮细胞表型的关键基因表达下调，例如E-钙粘蛋白（E-cadherin）。关键词：差异基因表达（Differential gene expression）；整体实验设计：本研究对小鼠晶状体设置三重生物学重复，共纳入3份野生型晶状体样本与3份Le-AP-2突变体样本。