miR-378a regulates keratinocyte responsiveness to IL-17A in psoriasis

In 2019, our group performed small RNA-sequencing on keratinocytes isolated from lesional and non-lesional psoriasis skin as well as from healthy skin, and identified miRNAs with altered levels in psoriasis keratinocytes (Srivastava et al., 2019). One of the miRNAs we identified to be overexpressed in psoriasis keratinocytes was miR-378a-3p. In this study, we aimed to explore the regulation and function of miR-378a in keratinocytes and its potential role in psoriasis. We used microarrays to identify differentially expressed genes upon miR-378a overexpression in primary human keratinocytes as part of the study and gain insights about the modulation of specific pathways. Transfected primary human keratinocytes overexpressing the microRNA miR-378a (treated or not with IL-17A) were collected, RNA was isolated and gene expression profiled by microarray analysis (Affymetrix Human Gene 2.0 ST Array)

2019年，本团队对从银屑病皮损区、非皮损区皮肤及健康皮肤中分离的角质形成细胞开展小RNA测序，筛选出在银屑病角质形成细胞中表达异常的微小RNA（microRNA，miRNA），相关研究成果发表于Srivastava等2019年的文献。其中，我们鉴定出的在银屑病角质形成细胞中高表达的miRNA之一为miR-378a-3p。
本研究旨在探究miR-378a在角质形成细胞中的调控机制与功能，及其在银屑病发病过程中的潜在作用。作为本研究的组成部分，我们通过微阵列技术筛选原代人角质形成细胞过表达miR-378a后的差异表达基因，以解析特定信号通路的调控模式。
我们收集了过表达miR-378a（经白细胞介素17A[IL-17A]处理或未处理）的转染原代人角质形成细胞，提取RNA并通过微阵列分析（Affymetrix Human Gene 2.0 ST Array）完成基因表达谱检测。