Characterising an extensive oak microbiota culture collection

A microbial culture collection of the oak microbiota, containing more than 30,000 bacterial and fungal isolates, was characterised using 16S rRNA gene and ITS amplicon sequencing. The composition of this culture collection (culture-dependent analysis) was then compared with the culture-independent microbiome profiles generated from the same tree tissue samples from which the isolates were obtained.In this data set, .qmd files contain the analysis of the oak microbial culture collection and the comparison between culture-dependent and culture-independent analyses of the oak microbiota. The numbers in the file names indicate the order in which the scripts should be run.The directory html_files contains the rendered HTML versions of the .qmd analyses.input_files.zip contains all RDS, CSV, and XLSX files required to run the .qmd scripts.A README.txt file is provided with a file description and software requirements.

本数据集涵盖橡树微生物群（oak microbiota）的微生物培养物库，该库收纳了逾30000株细菌与真菌分离株。研究采用16S rRNA基因（16S rRNA gene）与ITS扩增子测序（ITS amplicon sequencing）技术对该培养物库进行了表征。随后，将该培养物库的组成数据（即依赖培养的分析（culture-dependent analysis）结果），与从分离株来源的同一树木组织样本中获取的不依赖培养的微生物组谱（culture-independent microbiome profiles）进行对比分析。本数据集中的.qmd格式文件，包含橡树微生物培养物库的相关分析内容，以及橡树微生物群依赖培养与不依赖培养分析的对比研究；文件名中的数字标注了脚本的运行顺序。html_files文件夹内存储了所有.qmd分析文件渲染完成后的HTML版本。input_files.zip压缩包包含运行.qmd脚本所需的全部RDS、CSV及XLSX格式文件。数据集附带README.txt文件，其中说明了各文件的用途与运行所需的软件依赖。