_A_AgroinfFinalConstructsdCas9EV-GCMS.output.raw

pISA-tree file path: _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfFinalConstructsdCas9EV-GCMS\output\raw Title: Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the guide was made in pLX vector so it could be co-transformed in the same agrobacterium colony with the moth pheromone pathway. Such Agrobacterium containing both plasmids (guided/nonguided pathway + nonguided/guided copper-inducible dCas9EV) was agroinfiltrated before stable transformation in Nicotiana benthamiana to check the expression of the genes.pISA-tree 文件路径：_p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfFinalConstructsdCas9EV-GCMSoutput aw 标题：本氏烟草（Nicotiana benthamiana）叶片中铜诱导通路的最终构建体农杆菌（Agrobacterium）浸润实验 描述：本研究构建了两种重组载体，其一包含在T11中开发的合成启动子调控下的蛾类信息素通路3个结构基因，搭载nptII筛选标记，并分别携带/不携带用于激活的gRNA1；与此同时，我们在pLX载体中构建了另一组重组载体，该载体包含铜诱导型dCas9EV系统，分别携带/不携带向导RNA，使其可与蛾类信息素通路载体共同转化至同一农杆菌菌落中。将同时携带两种质粒（向导/非向导通路 + 非向导/向导铜诱导型dCas9EV系统）的农杆菌，在本氏烟草稳定转化前进行农杆菌浸润处理，以检测目标基因的表达情况。