Paracetamol (Acetaminophen) Electron Diffraction (MicroED) Datasets - Glacios TEM with a CETA-D

Electron diffraction datasets collected from nanometer sized crystals of paracetamol (acetaminophen, isolated from Tylenol® using hot acetone and filtration) applied to an electron microscopy grid covered in a pure carbon film. Data were collected using a Thermo Fisher Scientific Glacios Transmission Electron Microscope operated at 200 kV and -193 C using the software package Leginon (Cheng, et. al. 2021). The beam diameter is ~600 nm and the total dose is ~3.2e-/A^2. The crystals were continuously rotated and diffraction data was collected with a CETA-D camera in rolling shutter mode. Data have been converted to SMV format with the addition of an offset value to remove negative pixel values. This offset value can be found in the image headers, along with a suggested pedestal value. An image of the crystal before centering on the small green dot is also included. Data from four crystals are provided along with data reduction results. If processing in DIALS, you will first want to install the proper format class: 

dxtbx.install_format -u https://raw.githubusercontent.com/dials/dxtbx_ED_formats/master/FormatSMVCetaD_TUI.py

本数据集为采集自对乙酰氨基酚（paracetamol, acetaminophen）纳米晶体的电子衍射数据集：该晶体通过热丙酮萃取、过滤自泰诺®（Tylenol®），并被负载于涂覆纯碳支撑膜的电子显微镜载网之上。

数据采集使用赛默飞世尔科技（Thermo Fisher Scientific）Glacios型透射电子显微镜，工作加速电压为200 kV，样品台温度维持在-193 ℃，配套采集软件为Leginon（Cheng等，2021）。

本次采集的电子束束斑直径约为600 nm，总电子剂量约为3.2 e⁻/Ų。实验过程中晶体持续旋转，采用CETA-D相机以卷帘快门模式采集衍射数据。

所有数据已转换为SMV格式，并通过添加偏移值以消除负像素值；该偏移值及推荐的基底值均可在图像头文件中获取。

本数据集还附带了未对准该小绿点前的晶体成像图，提供了四颗晶体的衍射数据及对应的数据约简结果。

若使用DIALS软件进行数据处理，请首先安装对应的格式类：
dxtbx.install_format -u https://raw.githubusercontent.com/dials/dials_ED_formats/master/FormatSMVCetaD_TUI.py