A universal protein ladder for standardisation of diverse FRET assays: Supplementary figures

Raw data and analysis for the supplementary figures of: E. R. Smith et al. "A universal protein ladder for standardisation of diverse FRET assays", 2026.
doi: https://doi.org/10.64898/2026.03.05.709886
This is a modular protein ladder designed to provide a predictable calibration curve. This enables interpolation between different experimental FRET modalities, including confocal smFRET, flow cytometry based-FRET and Fluorescence Lifetime Imaging Microscopy FRET (FLIM-FRET).
Each figure has its own folder and is then split by experimental type. Each subfolder contains a readme file to describe the files contained in the folder and the data they contain.

本数据集包含E. R. Smith等人2026年发表的学术论文《用于多种荧光共振能量转移（Fluorescence Resonance Energy Transfer, FRET）检测标准化的通用蛋白质梯》的补充图表配套原始数据与分析结果，论文DOI：https://doi.org/10.64898/2026.03.05.709886。
该模块化蛋白质梯旨在构建可预测的校准曲线，可实现不同实验类型FRET检测技术间的插值校准，涵盖共聚焦单分子FRET（confocal smFRET）、基于流式细胞术的FRET以及荧光寿命成像显微镜FRET（Fluorescence Lifetime Imaging Microscopy FRET, FLIM-FRET）。
每张补充图表均设有独立文件夹，并按实验类型进一步拆分。每个子文件夹均包含README文件，用于说明该文件夹内的文件及所含数据内容。