Additional file 12: of A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)

230 Genes with putative Ca2+-signaling related functions. 230 genes with putative Ca2+-signaling related functions were identified here with quantifiable expression levels in the pollen RNA-Seq data set. Of those 230, 40 genes (yellow highlights) showed HS-dependent changes (≥ 2-fold changes with adjusted p-value ≤0.01) in WT pollen. Similar HS-dependent changes were also observed for cngc16 mutant, with seven potential exceptions with lower degrees of significance shown in bold. a Ratio of expression between pollen and seedling is based on Loraine et al. 2013 (PMCID: PMC3668042 [22]). b A comparison is provided to HS-dependent changes observed for aerial parts of seedlings exposed to a one hour 38 °C HS based on publicly available microarray data in AtGenExpress, ( http://jsp.weigelworld.org/expviz/expviz.jsp ; Schmid et al. 2005 (PMID: 15806101 [33]). The log2-fold change was calculated based on means of normalized values for two heat-stressed seedlings compared to two non-stressed seedlings. (XLSX 15 kb)

本研究共鉴定得到230个具备推定钙信号转导（Ca2+-signaling）相关功能的基因。这些基因在花粉RNA测序（RNA-Seq）数据集内均具有可量化的表达水平。在上述230个基因中，有40个基因（以黄色高亮标注）在野生型（Wild Type, WT）花粉中呈现出热胁迫（Heat Shock, HS）依赖的表达变化，其表达变化倍数≥2倍且校正后p值≤0.01。在cngc16突变体中同样观测到了类似的热胁迫依赖表达变化，仅存在7个显著性水平较低的潜在例外，以粗体标注。
a 花粉与幼苗的表达比值参考自Loraine等（2013）的研究（PMCID: PMC3668042 [22]）。
b 本研究结合AtGenExpress数据库公开的微阵列数据，将本研究结果与暴露于1小时38℃热胁迫的幼苗地上部分的热胁迫依赖表达变化进行了对比（相关链接：http://jsp.weigelworld.org/expviz/expviz.jsp；Schmid等，2005，PMID: 15806101 [33]）。其中，log2倍变化值基于2份热胁迫幼苗与2份非胁迫幼苗的标准化表达均值计算得到。
(XLSX 15 kb)