Transcriptomic changes mediated by β-amyloid in human aortic endothelial cells (HAOEC). Homo sapiens

We found that β-amyloid accumulation is modulated in HAOEC cells by overexpression or blocking of lncRNA BACE1-AS, which in turn regulates both BACE1 mRNA and protein expression. BACE1 is key-enzyme in the synthesis of β-amyloid from Amyloid Precursor Protein (APP). The transcriptomic changes mediated by 400nM β-amyloid was investigated in HAOEC cells. Overall design: HAOEC cells stimulated or not with 400nM β-amyloid

我们发现，在HAOEC细胞中，长链非编码RNA（lncRNA）BACE1-AS的过表达或沉默可调控β淀粉样蛋白（β-amyloid）的积累，而该调控作用可同时影响β位淀粉样前体蛋白裂解酶1（BACE1）的mRNA与蛋白表达水平。BACE1是从淀粉样前体蛋白（APP）合成β淀粉样蛋白的关键酶。本研究在HAOEC细胞中探究了400nM β淀粉样蛋白介导的转录组变化。实验整体设计：将HAOEC细胞分为两组，一组经400nM β淀粉样蛋白刺激，另一组不予处理。