Data for a publication "Biohastalex modified with silver nanolayers and heat treatment for antibacterial properties"

Abstract Here, we present surface analysis and biocompatibility evaluation of novel composite material based on graphene oxide traded as BioHastalex. The pristine material's surface morphology and surface chemistry were examined by various analytical methods. The BioHastalex with a thin silver layer was subsequently heat treated and characterized, the impact on the material surface wettability and morphology was evaluated. Significant surface roughness and morphology changes were detected at the nanometer scale after heat treatment of Ag-sputtered Biohastalex. The deposition of a thin silver nanolayer had an outstanding effect on Biohastalex's antibacterial properties while still maintaining cell viability (MRC-5, HaCaT). The heat treatment of Biohastalex-Ag led to the formation of regular nanocluster arrays while affecting the Ag concentration on the very surface. The decrease in silver concentration was connected with the length of heat treatment; cells growing on such samples exhibited good viability, and the antibacterial properties were weaker than simply sputtered Biohastalex.摘要 本研究针对以氧化石墨烯（graphene oxide）为基底的新型商用复合材料BioHastalex开展表面分析与生物相容性评价。研究团队采用多种分析手段对原始未处理材料的表面形貌与表面化学性质进行了表征。随后对镀有薄银层的BioHastalex进行热处理与表征，并探究了该处理对材料表面润湿性与形貌的影响。对溅射镀银的BioHastalex实施热处理后，在纳米尺度下观测到显著的表面粗糙度与形貌变化。薄银纳米层的沉积可显著提升BioHastalex的抗菌性能，同时仍能维持细胞活力（MRC-5、HaCaT细胞系）。对BioHastalex-Ag进行热处理可形成规则的纳米团簇阵列，同时会改变材料表层的银元素浓度。银元素浓度的降低与热处理时长呈正相关；在此类样品上培养的细胞仍表现出良好的活力，但其抗菌性能弱于仅经过溅射镀银的BioHastalex。