Normalized activity and affinity of β-Gal immobilized on surface-modified microwellsa.

aTypes of surfaces: 1 and 2 are selected peptide-modified surfaces; 3 and 4 are control surfaces modified by inhibitory peptides; 5 is a control surface conjugated with a weak-binding peptide; 6–8 are the conventional surfaces used for covalent or noncovalent enzyme immobilization, defined as in Figure 1, legend. All of the data is normalized to that of the amine surface, 8.表面类型说明如下：1号与2号为筛选得到的肽修饰表面；3号与4号为经抑制性肽修饰的对照表面；5号为结合弱结合肽的对照表面；6号至8号为用于共价或非共价酶固定化的常规表面，其定义详见图1图例。所有数据均以8号胺修饰表面的数据为基准进行归一化处理。