Brain tissue Protein Profile of Periplaneta americana exposed to commercial pesticide

Various organic and natural pesticides play a vital role in insect control, leading to variation in their protein profile (Abubakar et al., 2019; James & Xu, 2012). The sex, size, weight, and growth conditions could be the possible factors associated with fluctuations in the concentration of protein production in the cockroach (Bouchebtiet al., 2022) The current study of Perplanata americana under pesticide activity considered to observe the proteomics of Brain and protein expression under stressed conditions. 15 cockroaches were exposed to 5g of Laxman Rekha (11:1 ratio) to assess the impact of pesticide on protein synthesis. National and international research conducted on aqueous and alcoholic extraction of gut, exoskeleton and brain protein of Periplaneta spp. and Blatta spp have revealed 10 potential antibacterial proteins between 18 kDa to 72kDa range. These proteins were purified using chromatographic techniques like single dimension reducing PAGE, FPLC-MS, LC-MS, MALDI TOF-MS etc. The current study is aimed at isolation, identification and characterisation of proteins from cockroach brain tissue lysate using Liquid chromatography and Orbitrap Mass spectrometer technique. The data analysis was done using Thermo Proteome Discoverer software version 2.2. 0.388. The database used was Uniprot-Periplaneta americana.

各类有机及天然农药在害虫防治中发挥关键作用，其施用会导致昆虫蛋白质组谱发生改变（Abubakar等人，2019；James与Xu，2012）。性别、体型、体重及生长环境，可能是蜚蠊体内蛋白质合成浓度波动的潜在影响因素（Bouchebti等人，2022）。本研究以农药胁迫下的美洲大蠊（Perplaneta americana）为对象，旨在观察其脑部蛋白质组学特征及胁迫条件下的蛋白质表达模式。选取15只蜚蠊，暴露于5g配比为11:1的Laxman Rekha药剂中，以评估该农药对蛋白质合成的影响。针对蜚蠊属（Periplaneta spp.）和真蜚蠊属（Blatta spp.）的肠道、外骨骼及脑部蛋白质进行水提和醇提的国内外研究，已筛选出10种分子量介于18 kDa至72 kDa之间的潜在抗菌蛋白质。此类蛋白质可通过一维还原型聚丙烯酰胺凝胶电泳（single dimension reducing PAGE）、快速蛋白液相色谱-质谱联用（FPLC-MS）、液相色谱-质谱联用（LC-MS）、基质辅助激光解吸电离飞行时间质谱（MALDI TOF-MS）等色谱技术完成纯化。本研究拟采用液相色谱-轨道阱质谱联用技术，对蜚蠊脑组织裂解液中的蛋白质进行分离、鉴定与表征。数据分析采用赛默飞Proteome Discoverer软件2.2.0.388版本完成，所用数据库为Uniprot数据库美洲大蠊专属数据集。