Expression profiles of field-grown wheat expressing the Pm3 allele and null segregants. Triticum aestivum

It has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile study using GeneChip® Wheat Genome Array was performed to obtain insights into the mode of action of Pm3b and to elucidate the molecular basis of pleiotropic effects observed in three out of four independent transgenic events tested under field conditions. Overall design: 24 samples were analyzed. Three biological replicates of each sample were included. Two independent transformation events and respective null segregants as control treated with fungicide or artificially inoculated with powdery mildew.

已有研究证实，过表达R基因Pm3等位基因Pm3b的小麦，在田间条件下对白粉病的抗性显著增强。本研究采用GeneChip®小麦基因组芯片（GeneChip® Wheat Genome Array）开展基因表达谱分析，以期深入解析Pm3b的作用模式，并阐明经田间试验检测的4个独立转基因事件中3个所出现的多效性效应的分子基础。实验整体设计：共分析24份样本，每份样本设置3次生物学重复。实验包含2个独立转基因事件及其对应的无效分离株作为对照，对照组分别经杀菌剂处理或人工接种白粉病。