Gene expression during Bacillus subtilis spore germination and outgrowth (t=0-100). Bacillus subtilis subsp. subtilis str. 168

Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we studied this process in a time-dependent manner by a combination of microscopy, analysis of extracellular metabolites and a genome-wide analysis of transcription. The results indicate the presence of abundant levels of late sporulation transcripts in dormant spores. In addition, results suggest the existence of a complex and well-regulated spore outgrowth program, involving the temporal expression of at least 30 % of the B. subtilis genome. Keywords: time course, spore outgrowth Overall design: Time series of outgrowing B subtilis spores (cy-3) with common reference (RNA vegetative cells, TSB OD 0.4, Cy-5). Quality of the spotted oligoarray allows single dye dataanalysis.

枯草芽孢杆菌（Bacillus subtilis）在营养匮乏条件下会形成休眠孢子。当环境条件适宜时，孢子会打破休眠并恢复生长，该过程被称为孢子萌发与出芽。为阐明休眠孢子向活跃生长的营养细胞转变过程中涉及的生理过程，本研究结合显微镜技术、细胞外代谢物分析以及全基因组转录分析，以时间依赖性方式对该过程进行了研究。结果表明，休眠孢子中存在大量晚期芽孢形成转录本；此外，研究结果提示存在一套复杂且调控严谨的芽孢出芽程序，该程序涉及枯草芽孢杆菌基因组中至少30%的基因的时序性表达。
关键词：时间进程，芽孢出芽
整体实验设计：枯草芽孢杆菌出芽孢子（cy-3）的时间序列样本，以共同参考样本（营养体细胞RNA，TSB培养基培养至OD值0.4，Cy-5）作为对照。斑点寡核苷酸微阵列的质量可支持单染料数据分析。