Arabidopsis <i>PDS5</i> genes play pivotal roles in genome organization via suppressing TAD formation

Eukaryote genomes in interphase nuclei are organized in a non-random, hierarchical fashion that correlates to gene expression and other biological activities in the nucleus. One key structural element in the three-dimensional (3D) chromatin organization is the Topologically Associating Domain (TAD), which facilitates the creation of distinct chromatin compartments and specific chromatin interactions, including those between enhancers and their target genes. Similar chromatin compartments, known as TAD-like domains, have been identified in many plant species. However, they have been curiously sparse in the model plant Arabidopsis thaliana. In our recent research, we observed substantial formation of TAD-like domains in an Arabidopsis mutant plant. This webpage contains various types of datasets supporting our conclusion. Please check "Figshare_file_description.xlsx" for details of individual files.间期细胞核内的真核生物基因组呈现非随机的层级化排布模式，该模式与细胞核内的基因表达及其他生物学活动密切相关。三维（3D）染色质组织的关键结构元件之一为拓扑关联结构域（Topologically Associating Domain，TAD），其可介导形成独特的染色质区室与特异性染色质相互作用，包括增强子与其靶基因之间的相互作用。在诸多植物物种中均已鉴定出类似的染色质区室，即类TAD结构域。但令人费解的是，模式植物拟南芥中此类结构却极为稀少。在我们近期的研究中，于一株拟南芥突变体中观察到了大量类TAD结构的形成。本网页包含支撑该研究结论的多类数据集，各文件的详细信息请参阅"Figshare_file_description.xlsx"。