mRNA expression profiles of oxygen-induced retinopathy (OIR) mouse retinas after knockdown treatment of lncRNA-MM2P. mRNA expression profiles of oxygen-induced retinopathy (OIR) mouse retinas after knockdown treatment of lncRNA-MM2P

The purpose of this study is to investigate the mRNA expression profiles of OIR retinas after knockdown treatment of lncRNA-MM2P. We established an OIR model in C57BL/6J mice. The expression of lncRNA-MM2P in the retinas of OIR mice was knocked down by intravitreal injection of self-complementary adeno-associated virus (scAAV). Retinas of four eyes from two OIR mice were collected as one sample. The control group was treated with control scAAV and named as AAV_NC, the knockdown group was treated with knockdown scAAV and named as AAV_sh. The total RNA was extracted from total of eight samples (four samples each group) for RNA sequencing. Overall design: RNA sequencing of retinas from 17-day old oxygen-induced retinopathy (OIR) C57BL/6J mice which were knocked down of lncRNA-MM2P by scAAV or treated with control scAAV.

本研究旨在探究长链非编码RNA-MM2P（lncRNA-MM2P）敲低处理后，氧诱导视网膜病变（Oxygen-induced retinopathy, OIR）小鼠视网膜的mRNA表达谱。本研究在C57BL/6J小鼠中构建了OIR模型，通过玻璃体内注射自互补腺相关病毒（self-complementary adeno-associated virus, scAAV）敲低OIR小鼠视网膜内的lncRNA-MM2P表达。每2只OIR小鼠的4只眼视网膜作为一个样本，共设置两组：对照组注射对照scAAV，命名为AAV_NC；敲低组注射敲低专用scAAV，命名为AAV_sh。随后从两组各4个样本（共8个样本）中提取总RNA进行RNA测序。整体实验设计：对通过scAAV敲低lncRNA-MM2P或注射对照scAAV的17日龄C57BL/6J OIR小鼠的视网膜进行RNA测序。