Plant Hormones and Volatiles Response to Temperature Stress in Sweet Corn (Zea mays L.) Seedlings

This
work aims to emphasize on disclosing the regulative mechanism
of sweet corn seedlings response to extreme temperature stress; transcriptomics
and metabolomics for volatiles and plant hormones were integrated
in this study. Results showed that low-temperature stress significantly
impressed 20 volatiles; abscisic acid and salicylic acid accumulated,
while auxin and jasmonic acid decreased. The regulatory patterns of vp14 and ABF for abscisic acid accumulation
and signal transduction were elucidated in low-temperature stress.
High-temperature stress influenced 31 volatiles and caused the reductions
on zeatin, salicylic acid, jasmonic acid, and auxin. The up-regulation
of an ARR-B gene emphasized its function on zeatin
signal transduction under high-temperature stress. Correlations among
gene modules, phytohormones, and volatiles were analyzed for building
the regulative network of sweet corn seedlings under temperature stress.
The attained result might build foundations for improving early development
of sweet corn by biological intervention or genomic-level modulation.

本研究旨在揭示甜玉米幼苗响应极端温度胁迫的调控机制。本研究整合了针对挥发性物质(volatiles)与植物激素(plant hormones)的转录组学(transcriptomics)和代谢组学(metabolomics)分析。结果显示：低温胁迫显著影响了20种挥发性物质的积累；脱落酸(abscisic acid)与水杨酸(salicylic acid)含量上升，而生长素(auxin)和茉莉酸(jasmonic acid)含量下降。本研究阐明了低温胁迫下VP14与ABF在脱落酸积累及信号转导中的调控模式。高温胁迫影响了31种挥发性物质，并导致玉米素(zeatin)、水杨酸、茉莉酸及生长素含量降低。一个ARR-B型基因(ARR-B)的上调表达，凸显了其在高温胁迫下玉米素信号转导中的功能。本研究通过分析基因模块、植物激素与挥发性物质间的关联，构建了甜玉米幼苗在温度胁迫下的调控网络。本研究所得结果可为通过生物干预或基因组水平调控手段改良甜玉米早期生长发育奠定基础。