Supplementary Material for: Protein analysis of the surface of orthokeratology lenses

Introduction
Overnight orthokeratology (OK) lenses control myopia in children, but the underlying mechanism is unclear. We hypothesized that ocular surface proteins might deposit directly on the surface of OK lenses during wear. This could offer a non-invasive method for investigating the potential molecular mechanisms behind the myopia control effect of OK lenses.

Case Presentation
A healthy 30 year old male without prior contact lens wear history wore bespoke OK lenses overnight. After lens removal, proteomic analysis was performed using nano liquid chromatography tandem mass spectrometry to identify proteins on the surface of the lenses. Proteomic analysis identified 423 proteins on the right OK lens and 303 on the left, with 273 proteins common to both lenses. The proteins originated from various cellular compartments.

Conclusion
Ocular surface proteins can be detected directly from OK lenses, offering a novel approach to studying the molecular mechanisms of myopia control.

引言
夜间配戴角膜塑形镜（orthokeratology, OK）可控制儿童近视，但其潜在作用机制尚不明确。本研究假设，配戴过程中眼表蛋白可能直接沉积于角膜塑形镜表面，这可为探究角膜塑形镜控制近视效应背后的潜在分子机制提供一种非侵入性方法。

病例介绍
1名既往无角膜接触镜配戴史的健康30岁男性，夜间配戴定制化角膜塑形镜。摘镜后，采用纳米液相色谱-串联质谱（nano liquid chromatography tandem mass spectrometry）进行蛋白质组学分析（proteomic analysis），以鉴定镜片表面的蛋白。蛋白质组学分析结果显示，右眼配戴的角膜塑形镜表面检出423种蛋白，左眼配戴的角膜塑形镜表面检出303种，其中273种为双侧镜片共有。这些蛋白源自多种细胞区域。

结论
可直接从角膜塑形镜表面检出眼表蛋白，为研究近视控制的分子机制提供了全新途径。