RNA-seq of Pacific oyster Crassostrea gigas under different developmental stages and stress treatments

Deep sequencing of samples from different development stages, different adult organs and different stress treatments of Pacific oyster Crassostrea gigas Overall design: Samples of 38 developmental stages from egg to juvenile were analyzed using single-end 49 bp RNA-seq. Two libraries mixed by RNAs from different developmental stages were analyzed using paired-end 90 bp RNA-seq. A total of 11 samples mainly from 8 organs (mantle, gill,adductor muscle, digestive gland, hemocyte, labial palp, female gonad and male gonad) were analyzed using paired-end 90 bp RNA-seq. At the same time, single-end 49 bp RNA-seq was conducted on 61 samples collected from adult oysters subjected to 9 types of environmental stressors (exposure to air, salinity, temperature, and exposure to metals).

太平洋牡蛎（Pacific oyster，学名Crassostrea gigas）不同发育阶段、不同成体组织以及不同胁迫处理样本的深度测序研究。整体实验设计如下：对从卵到稚贝的38个发育阶段样本进行单端49 bp RNA测序（RNA-seq）；构建了2个由不同发育阶段RNA混合而成的文库，并对其开展双端90 bp RNA测序（RNA-seq）；共计获取11个样本进行双端90 bp RNA测序（RNA-seq），这些样本主要源自8种成体组织：外套膜（mantle）、鳃（gill）、闭壳肌（adductor muscle）、消化腺（digestive gland）、血淋巴细胞（hemocyte）、唇瓣（labial palp）、雌性性腺（female gonad）与雄性性腺（male gonad）；同时，对取自经9种环境胁迫处理的成体牡蛎的61个样本开展单端49 bp RNA测序（RNA-seq），胁迫处理类型涵盖空气暴露、盐度胁迫、温度胁迫及金属暴露。