Establishment of a refined oral glucose tolerance test in pigs, and assessment of insulin, glucagon and glucagon-like peptide-1 responses

Diabetes mellitus is increasing worldwide and reliable animal models are important for progression of the research field. The pig is a commonly used large animal model in diabetes research and the present study aimed to refine a model for oral glucose tolerance test (OGTT) in young growing pigs, as well as describing intravenous glucose tolerance test (IVGTT) in the same age group. The refined porcine OGTT will reflect that used in children and adolescents. Eighteen pigs were obtained one week after weaning and trained for two weeks to bottle-feed glucose solution, mimicking the human OGTT. The pigs subsequently underwent OGTT (1.75 g/kg BW) and IVGTT (0.5 g/kg BW). Blood samples were collected from indwelling vein catheters for measurements of glucose and the diabetes related hormones insulin, glucagon and active glucagon-like peptide-1. The study confirmed that pigs can be trained to bottle-feed glucose dissolved in water and thereby undergo an OGTT more similar to the human standard...

糖尿病在全球范围内发病率持续升高，可靠的动物模型对该研究领域的推进具有重要意义。猪是糖尿病研究中常用的大型动物模型，本研究旨在优化青年生长猪的口服葡萄糖耐量试验（oral glucose tolerance test, OGTT）模型，并描述同年龄段猪的静脉葡萄糖耐量试验（intravenous glucose tolerance test, IVGTT）方法。经优化的猪口服葡萄糖耐量试验方案将更贴合儿童与青少年的临床检测流程。本研究纳入18头断奶1周后的仔猪，经过2周训练使其能够通过奶瓶摄取葡萄糖溶液，以模拟人类口服葡萄糖耐量试验的操作流程。随后对这些仔猪分别实施口服葡萄糖耐量试验（给药剂量为1.75 g/kg体重）与静脉葡萄糖耐量试验（给药剂量为0.5 g/kg体重）。通过留置静脉导管采集血液样本，用于检测血糖以及糖尿病相关激素：胰岛素、胰高血糖素与活性胰高血糖素样肽-1。本研究证实，可通过训练使仔猪摄取溶于水的葡萄糖，从而开展更贴近人类标准的口服葡萄糖耐量试验……