Microsatellite genotypes and partial COI fragments for 5 cod species in the Chukchi Sea, Arctic Integrated Ecosystem Research Program, 2017

These data are part of the Arctic IES component of the Arctic Integrated Ecosystem Research Program (IERP), sponsored by the North Pacific Research Board (NPRB), Collaborative Alaskan Arctic Studies Program (formerly the North Slope Borough/Shell Baseline Studies Program), Bureau of Ocean Energy Management (BOEM), and the Office of Naval Research Marine Mammals and Biology Program. Initially, Arctic Cod (Boreogadus saida) was collected from the Chukchi Sea for genetic population structure analyses. Bulk samples of approximately 50 fish were retained from each of 12 midwater trawls (n=594). Samples were collected in August and September, 2017, for species identification. These samples were examined at 15 microsatellite loci and genotypes were recorded in an excel spreadsheet (two fragment sizes for each specimen). Additionally, phase 2 of the project was initiatiated, because the microsatellite analysis detected other cod species in the Arctic Cod sample. Fin clips from other cod projects were collected for genetic analysis to verify species identification (n=1545). mtDNA was examined at a partial (100 base pair) COI fragment and aligned with known sequences from other possible cods. Sequences are stored in a text file.

本数据集隶属于北极综合生态系统研究计划（Arctic Integrated Ecosystem Research Program, IERP）的北极IES组分，由北太平洋研究委员会（North Pacific Research Board, NPRB）、阿拉斯加北极合作研究计划（前身为北坡自治市/壳牌基线研究计划）、海洋能源管理局（Bureau of Ocean Energy Management, BOEM）以及海军研究办公室海洋哺乳动物与生物学项目资助。最初，研究人员从楚科奇海采集北极鳕（Boreogadus saida）样本以开展遗传种群结构分析。从12次中层拖网作业中各保留约50尾鱼类的混合样本，总样本量为594尾（n=594）。所有样本于2017年8月至9月间采集，用于物种鉴定。研究人员对这批样本的15个微卫星位点（microsatellite loci）进行基因分型检测，并将基因型数据记录于Excel电子表格中（每个样本对应两个片段长度值）。此外，由于微卫星分析在北极鳕样本中检出了其他鳕科物种，项目第二阶段正式启动。研究人员收集了其他鳕鱼研究项目的鳍剪样本用于遗传分析，以验证物种鉴定，总样本量达1545份（n=1545）。对线粒体DNA（mtDNA）的部分COI基因片段（长度为100碱基对）进行测序，并与其他潜在鳕科物种的已知序列进行序列比对。所有测序序列均存储于文本文件中。