Single-Cell RNAsequencing of Human Naïve Pluripotent Stem Cells to Trophoblast Stem Cells with and without EZH2 inhibition.. Single-Cell RNAsequencing of Human Naïve Pluripotent Stem Cells to Trophoblast Stem Cells with and without EZH2 inhibition.

Single-Cell RNAsequencing of the conversion of naïve iCas9 Sigma hiPSC line 1 (iCSIG-1 hiPSCs) into trophoblast stem cells with and without EZH2i (Abcam ab146152 UNC1999, EZH2 methyltransferase inhibitor) during 4 days preconversion and during 4 days during conversion (total of 8 days with or without PRC2i). Overall design: Examination of the inhibition of EZH2 in human naïve pluripotent stem cells and day 4 of the conversion of naïve into trophoblast stem cells.

本数据集为单细胞RNA测序数据，聚焦初始态iCas9 Sigma人类诱导多能干细胞系1（iCSIG-1 hiPSCs, human induced pluripotent stem cells）在添加或不添加EZH2抑制剂（Abcam公司ab146152号产品UNC1999，一种EZH2甲基转移酶抑制剂）的条件下，于预转化4天及转化过程中4天（总计8天，含或不含多梳抑制复合体2抑制剂PRC2i, polycomb repressive complex 2 inhibitor）向滋养层干细胞（trophoblast stem cells）转化的过程。实验整体设计为：探究EZH2抑制对人类初始态多能干细胞，以及初始态多能干细胞向滋养层干细胞转化第4天样本的生物学影响。