Synergy between spores and S-layers against Culex sp. Larvae.

S-layer and spores preparations were added separately or mixed at sub-lethal concentrations with 20 Culex sp. larvae as indicated in Material and Methods. After 24 h lethality was evaluated. Only the % of died larvae are reported. The concentration of spores or S-layer protein individually or together per experiment were 500 CFU/ml and 0.25 µg for 2362 strain and 50 CFU/ml and 0.6 µg for C7 strain.Synergy between spores and S-layers against Culex sp. Larvae.S层（S-layer）与芽孢制剂可单独添加，或以亚致死浓度混合后作用于20只库蚊属（Culex sp.）幼虫，具体操作详见材料与方法。于24小时后评估幼虫致死效应，仅统计死亡幼虫所占百分比。各实验中，芽孢或S层蛋白单独或联合使用的浓度设置如下：2362菌株对应500 CFU/ml与0.25 µg，C7菌株对应50 CFU/ml与0.6 µg。芽孢与S层对库蚊属幼虫的协同灭杀作用。