Analytical ultracentrifugation datasets of NT-2Rep-CT

AUC datasets for "Metastable liquid-liquid phase separation and aging lead to strong processing path dependence in mini-spidroin solutions" publication.

Dataset RunId 861 corresponds to Fig. 2a and Fig. 4a and contains information about NT-2Rep-CT sample 20 g/l concentration in 20 mM Tris pH 8.0 and 110 mM Tris pH 7.2 buffers.

Datasets RunId 989 – 1032 correspond to Fig. 3.

RunId 989, 991, 1000, 1018 and 1026 were measured using different protein concentrations: 989: 0.675, 1.25 and 2.5 g/l; 991: 10, 5 and 20 g/l; 1000: 0.1 and 2.5 g/l.

Datasets RunId 1018 – 1024 contain information about time dependence of NT-2Rep-CT sample at 0.05, 0.1 and 1 g/l concentration in 20 mM Tris pH 8.0 buffer. RunId 1018 – first experiment (0 hour), RunId 1020 – after 24 hours, RunId 1022 – after 48 hours and RunId 1024 – after 120 hours.

Datasets RunId 1026 – 1032 contain information about time dependence of NT-2Rep-CT sample at 1, 5 and 10 g/l concentration in 20 mM Tris pH 8.0 buffer. RunId 1026 – first experiment (0 hour), RunId 1028 – after 24 hours, RunId 1030 – after 48 hours and RunId 1032 – after 120 hours.

本数据集为发表于论文《亚稳态液液相分离与陈化使微型蛛丝蛋白溶液呈现显著加工路径依赖性》（Metastable liquid-liquid phase separation and aging lead to strong processing path dependence in mini-spidroin solutions）的分析超速离心（Analytical Ultracentrifugation, AUC）数据集。
运行编号（RunId）861对应图2a与图4a，包含NT-2Rep-CT样品在20 mM 三羟甲基氨基甲烷（Tris）缓冲液（pH 8.0）及110 mM Tris缓冲液（pH 7.2）中、浓度为20 g/l的相关实验数据。
运行编号989至1032对应图3。
运行编号989、991、1000、1018及1026采用不同蛋白浓度开展测试：其中RunId 989的蛋白浓度为0.675、1.25与2.5 g/l；RunId 991为10、5与20 g/l；RunId 1000为0.1与2.5 g/l。
运行编号1018至1024包含NT-2Rep-CT样品在20 mM Tris缓冲液（pH 8.0）中、浓度为0.05、0.1及1 g/l时的时间依赖性实验数据。其中RunId 1018为首次实验（0小时），RunId 1020为静置24小时后，RunId 1022为静置48小时后，RunId 1024为静置120小时后。
运行编号1026至1032包含NT-2Rep-CT样品在20 mM Tris缓冲液（pH 8.0）中、浓度为1、5及10 g/l时的时间依赖性实验数据。其中RunId 1026为首次实验（0小时），RunId 1028为静置24小时后，RunId 1030为静置48小时后，RunId 1032为静置120小时后。