Translational and Transcriptional control of gene expression during serum stimulation on human RPE-1 cells

In animal cells, growth factor signaling promotes both cell cycle progression and cell growth. To understand how the cell cycle is coupled to growth, we examined translational activities in human Retinal Pigment Epithelial cells by whole-transcriptome ribosome profiling. We performed Ribo-Seq and RNA-Seq on human RPE-1 cells that are serum starved for 18h, then serum stimulated for 5h, 10h, and 15h with 4 biological replicates each.

在动物细胞中，生长因子信号通路可同时促进细胞周期进程与细胞生长。为阐明细胞周期与细胞生长的耦合调控机制，我们借助全转录组核糖体谱分析（whole-transcriptome ribosome profiling），对人类视网膜色素上皮细胞的翻译活性展开了检测。本研究对经历18小时血清饥饿、随后分别以5小时、10小时、15小时血清刺激的人类RPE-1细胞进行了核糖体测序（Ribo-Seq）与RNA测序（RNA-Seq），每组设置4次生物学重复。