Quantitative RT-PCR for neuropeptide and energy sensing genes in hypothalamus.

Quantitative RT-PCR for selected genes was performed on twelve hypothalamus samples per condition, reactions were done in triplicates. Delta Ct values were determined relative to the Ct value for Ppia in the same sample. P-values were obtained by performing two-tailed Student's t-test on delta Ct values.

针对选定基因的定量实时逆转录聚合酶链反应（Quantitative RT-PCR）在各实验条件下的12份下丘脑样本中开展，所有反应均设置三次重复。以同一样本中Ppia的Ct值为参照，计算得到ΔCt值。通过对ΔCt值执行双侧学生t检验，获得相应的P值。