Gene expression in astrocytes upon sustained GSK-3 inhibition by small molecule

In order to identify molecular targets that mediates the stimulation of astrocyte's migration upon sustained specific inhibition of GSK-3, we have employed whole genome microarray expression profiling. Murine cortical-striatal astrocytes grown in primary culture were treated in vitro for 48 h with either 1 micromolar Ro3303544, or control medium (final equivalent concentration of DMSO), or washed-out for 48h in control medium after an initial 48h treatment with 1 micromolar Ro3303544. The three different conditions: Ctrl, Ro and wash-out (each in duplicate) were compared.

为鉴定介导持续特异性抑制糖原合成激酶3（GSK-3）后星形胶质细胞迁移刺激效应的分子靶点，我们采用了全基因组微阵列表达谱分析（whole genome microarray expression profiling）技术。将原代培养的小鼠皮质-纹状体星形胶质细胞开展体外处理实验，设置三种不同条件：以1 μM Ro3303544处理48小时；以含终浓度等效二甲基亚砜（DMSO）的对照培养基处理；先以1 μM Ro3303544处理48小时，随后更换为对照培养基洗脱培养48小时。每组均设置生物学重复两份，最终对对照组（Ctrl）、Ro处理组（Ro）及洗脱组（wash-out）三种处理条件进行比较分析。