The Aquatic eDNAtlas Project: Lab Results Map - USFS RMRS

Aquatic environmental DNA (eDNA) sampling is the collection of DNA released by a target species into streams, rivers, ponds, lakes, and wetlands. Detection of stream fish with eDNA can be remarkably sensitive—100% detection efficiency of target species has been achieved despite order-of-magnitude changes in stream discharge. The eDNA samples in the eDNAtlas database describe species occurrence locations and were collected by the U.S. Forest Service and numerous agencies that have partnered with the National Genomics Center for Wildlife and Fish Conservation (NGC) throughout the United States. The data were collected for a variety of project-specific purposes that included: species status assessments, trend monitoring at one or many sites, development of predictive species distribution models, detection and tracking of non-native species invasions, and assessments of habitat restoration efforts. The eDNAtlas database consists of two feature classes. The first component (eDNAtlas_West_AGOL_ResultsOnly) is a database of georeferenced species occurrence locations based on eDNA field sampling results, which are downloadable by species through a dynamic ArcGIS Online (AGOL) mapping tool. The earliest eDNA samples in the database were collected in 2015 but new samples and results are added annually to the database, which houses thousands of species occurrence records. The second component (eDNAtlas_West_SampleGridAndResults) is a systematically-spaced 1-kilometer grid of potential sample points in streams and rivers throughout the western United States. Future versions will include the eastern United States as well. The points in the sampling grid are arrayed along the medium-resolution National Hydrography Dataset Version 2 (NHDPlusV2) and can be used to develop custom eDNA sampling strategies for many purposes. Each sample point has a unique identity code that enables efficient integration of processed eDNA sample results with the species occurrence database.
The eDNAtlas is accessed via an interactive ArcGIS Online (AGOL) map that allows users to view and download sample site information and lab results of species occurrence for the U.S. The results are primarily based on samples analyzed at the National Genomics Center for Wildlife and Fish Conservation (NGC) and associated with geospatial attributes created by the Boise Spatial Streams Group (BSSG).
The AGOL map displays results for all species sampled within an 8-digit USGS hydrologic unit or series of units. The map initially opens to the project extent, but allows users to zoom to areas of interest. Symbols indicate whether a field sample has been collected and processed at a specific location, and if the latter, whether the target species was present. Each flowing-water site is assigned a unique identification code in the database to ensure that it can be tracked and matched to geospatial habitat descriptors or other attributes for subsequent analyses and reports. Because no comparable database has been built for standing water, results for those sites lack this additional information but still provide data on the sample and species detected. Resources in this dataset:Resource Title: The Aquatic eDNAtlas Project: Lab Results Map - USFS RMRS. File Name: Web Page, url: https://usfs.maps.arcgis.com/apps/webappviewer/index.html?id=b496812d1a8847038687ff1328c481fa The eDNAtlas is accessed via an interactive ArcGIS Online (AGOL) map that allows users to view and download sample site information and lab results of species occurrence for the U.S. The results are primarily based on samples analyzed at the National Genomics Center for Wildlife and Fish Conservation (NGC) and associated with geospatial attributes created by the Boise Spatial Streams Group (BSSG).
The AGOL map displays results for all species sampled within an 8-digit USGS hydrologic unit or series of units. The map initially opens to the project extent, but allows users to zoom to areas of interest. Symbols indicate whether a field sample has been collected and processed at a specific location, and if the latter, whether the target species was present. Each flowing-water site is assigned a unique identification code in the database to ensure that it can be tracked and matched to geospatial habitat descriptors or other attributes for subsequent analyses and reports. Because no comparable database has been built for standing water, results for those sites lack this additional information but still provide data on the sample and species detected.
For details on using the map see the Aquatic eDNAtlas Project: Lab Results ArcGIS Online Map Guide.

水生环境DNA（eDNA）采样是指收集目标物种释放到溪流、河流、池塘、湖泊和湿地中的DNA。利用eDNA检测溪流中的鱼类具有极高的灵敏度——即使在溪流流量发生数量级变化的情况下，也能实现目标物种100%的检测效率。eDNAtlas数据库中的eDNA样本描述了物种的分布地点，并由美国森林管理局以及与美国国家野生动物和鱼类保护基因组中心（NGC）合作的众多机构收集。这些数据收集旨在满足多种特定项目需求，包括物种状况评估、一个或多个地点的趋势监测、预测物种分布模型的开发、外来物种入侵的检测与追踪，以及栖息地恢复努力的评估。eDNAtlas数据库由两个功能类别组成。第一个组成部分（eDNAtlas_West_AGOL_ResultsOnly）是基于eDNA现场采样结果的地理参照物种分布地点数据库，可通过动态ArcGIS在线（AGOL）地图工具按物种下载。数据库中最早的eDNA样本收集于2015年，但每年都会向数据库中添加新的样本和结果，其中包含数千个物种分布记录。第二个组成部分（eDNAtlas_West_SampleGridAndResults）是美国西部溪流和河流中系统间隔距为1公里的潜在样本点网格。未来版本将包括美国东部。采样网格中的点沿中等分辨率的全国水文数据集第二版（NHDPlusV2）排列，可用于开发针对多种目的的定制eDNA采样策略。每个样本点都有一个唯一的身份代码，这使得将处理后的eDNA样本结果与物种分布数据库进行高效集成成为可能。

eDNAtlas可通过交互式的ArcGIS在线（AGOL）地图访问，允许用户查看和下载美国样本地点信息和物种出现实验室结果。结果主要基于国家野生动物和鱼类保护基因组中心（NGC）分析的样本，并关联由博伊西空间溪流组（BSSG）创建的地理空间属性。

AGOL地图显示了一个8位USGS水文单元或一系列单元内所有被采样物种的结果。地图最初打开到项目范围，但允许用户放大到感兴趣的区域。符号指示是否在特定位置收集并处理了现场样本，以及如果处理了，目标物种是否出现。每个流动水地点在数据库中分配了一个唯一的识别代码，以确保它可以被追踪并与地理空间栖息地描述符或其他属性匹配，用于后续分析和报告。由于尚未为静水构建类似的数据库，因此这些地点的结果缺少此附加信息，但仍提供有关样本和检测到的物种的数据。

关于使用地图的详细信息，请参阅水生eDNAtlas项目：实验室结果ArcGIS在线地图指南。