Metagenome assembled genomes co_8-Bin_21_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.

采用CONCOCT工具在anvi’o v5.1平台中对共组装产物进行分箱：通过将长度≥2500 bp的支架序列（scaffold）聚类为宏基因组组装基因组（Metagenome Assembled Genomes，MAGs），并在anvi’o平台内对其进行手动优化。此外，使用dRep v2.3.2工具对MAGs进行去冗余处理，通过设置参数‘-comp 50 -con 10’并采用默认参数，获取代表性基因组。