Gene expression profile at single cell level of human nasal primary airway cells from DNAH5 subjects

DNAH5 is an cilia dynein motor . We used single cell RNA sequencing (scRNA-seq) to analyze the changes in cells types and gene expression profile of subjects with variants in DNAH5 compared to healthy individuals Overall design: Nasal cells were isolated and diffrentiated using Air-liquid phase conditions, and analyzed using scRNAseq.

DNAH5是一种纤毛动力蛋白马达。本研究采用单细胞RNA测序（scRNA-seq）技术，对比分析了携带DNAH5变异的受试者与健康个体的细胞类型变化及基因表达谱。
实验整体设计：分离鼻腔细胞并通过气液界面培养条件诱导其分化，随后采用单细胞RNA测序进行分析。