Macrophage response to extracellular ADP stimulation

The gene expression analysis of bone marrow-derived macrophages (BMDM) treated with 100uM ADP was employed. Extracellular ADP is a danger signal that induces immune responses. Results provide insight into macrophage functions altered in ADP stimulation. Extracellular ADP induced gene expression in BMDMs was measured at 6 hours after exposure to dose of 100uM ADP. Three independent experiments were performed at each time using different donors for each experiment.

本研究针对经100μM腺苷二磷酸（adenosine diphosphate, ADP）处理的骨髓来源巨噬细胞（bone marrow-derived macrophages, BMDM）开展基因表达分析。细胞外腺苷二磷酸是一类可诱导免疫应答的危险信号。本研究结果可为ADP刺激下功能发生改变的巨噬细胞的相关研究提供洞察。在将骨髓来源巨噬细胞暴露于100μM ADP环境中6小时后，我们对该细胞中由细胞外ADP诱导的基因表达水平进行了检测。本研究共开展三次独立实验，每次实验均使用不同供体的细胞。