Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells [ChIP-seq]

Human acute myeloid leukemia (AML) KG1a cells or mouse BM cells (mouse bone morrow cells were employed for each ChIP assay. The ChIP procedure was performed according to a previously described protocol (Lee et al., 2006; Ying et al., 2017), using anti-ATF4 antibody (Abcam) ChIP-sequencing (ChIP-seq) of ATF4 in human AML KG1a cells and mouse BM cells. Input samples included.

实验所用细胞包括人类急性髓系白血病（acute myeloid leukemia, AML）KG1a细胞与小鼠骨髓细胞，本研究中所有染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）实验均采用小鼠骨髓细胞。染色质免疫沉淀测序（ChIP-sequencing, ChIP-seq）实验依照已发表的实验方案（Lee等，2006；Ying等，2017）开展，使用抗ATF4抗体（Abcam），分别对人类AML KG1a细胞及小鼠骨髓细胞中的ATF4进行ChIP-seq检测，实验设置输入样本作为对照。