Inline holographic microscopy through a fibre imaging bundle Fig. 2

This dataset and code provides an example for reconstructing inline holography images through a fibre imaging bundle. The sample is 5 micron diameter microspheres on a 1 mm thick glass slide. The slide was back illuminated by 450 nm LED light coupled through a multimode fibre with a 50 micron diameter core. The illumination fiber was approximately 15 mm behind the slide. The hologram was collected by a Fujikura FIGH-30-650S fibre bundle approximately 0.5 mm in front of the microspheres. At the proximal end, this was imaged onto a CMOS camera via a 20X objective and a tube lens, giving an effective pixel size of 0.44 microns.

本数据集与配套代码提供了通过光纤成像束（fibre imaging bundle）重建同轴全息（inline holography）图像的示例。实验样本为置于1mm厚载玻片（glass slide）上、直径5微米的微球（microspheres）。载玻片采用450nm LED光进行背向照明，该光源通过纤芯直径50微米的多模光纤（multimode fibre）耦合接入。照明光纤位于载玻片后方约15mm处。全息图由藤仓（Fujikura）FIGH-30-650S型光纤成像束采集，该探头位于微球前方约0.5mm位置。在光纤束的近端，系统通过20倍物镜（objective）及镜筒透镜（tube lens）将光束成像至CMOS相机，最终获得有效像素尺寸为0.44微米的成像结果。