CIL:12090, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

During division the micronucleus contains internal microtubules as well as a layer of b-tubulin, not in microtubular form, on or just inside the nuclear envelope. The section is immunogold labeled with anti-b-tubulin. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 60kV. Neg. 19,800X. Bar = 0.2 µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:1314) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

细胞分裂过程中，微核（micronucleus）内部含有微管，同时在核被膜（nuclear envelope）表面或紧邻核被膜内侧存在一层非微管组装形式的β微管蛋白（β-tubulin）。该切片使用抗β微管蛋白抗体完成免疫金标记。
本图像由R. Allen于1996年7月8日使用加速电压为60kV的蔡司（Zeiss）10A型透射电子显微镜（Transmission Electron Microscope, TEM）拍摄，放大倍数为19800倍，标尺长度为0.2微米（µm）。
实验细胞先经0.25%戊二醛轻度固定，再以2.3M蔗糖进行渗透处理，随后置于液氮中快速冷冻，并在–100°C条件下进行超薄切片，切片厚度约为75纳米（nm）。
将上述制备得到的冰冻切片解冻、洗涤后，依次与小鼠、家兔或山羊源的单克隆一抗，以及偶联胶体金的山羊抗小鼠/兔二抗共同孵育。
样本制备的详细流程可参阅《Methods Cell Biol.》2010年第96卷第143至173页。
原始负片经打印后扫描导入Adobe Photoshop软件进行处理，本数字化图像可用于定性分析。该图像的未处理高分辨率版本（编号CIL:1314）馆藏于图像库中，可用于定量分析。更多相关信息可访问：http://www5.pbrc.hawaii.edu/allen/