Epigenome analysis of DNA modification 5-hydroxymethylcytosine in normal, abnormal, and globozoospermia sperm genomes. Homo sapiens

Genome wide of 5-hydroxymethylcytosine profiling of normal and abnormal, and globozoospermia sperm genomes.Two semen samples were collected from a healthy man and a globozoospermia patient who had consulted a physician at ShengJing Hospital of China Medical University. Other semen samples obtained from two volunteers who were good health generally. 5-hmC enriched genomic DNA libraries were generated following the Illumina protocol for “Preparing Samples for CHIP sequencing of DNA”. 100bp single end sequencing on Illumina Hiseq2000 to get 5-hmC-enriched DNA fragment sequence was performed. Overall design: Examination of 5hmC levels in normal, abnormal, and globozoospermia sperm genomes

本数据集针对正常、异常及圆头精子症（globozoospermia）精子基因组的5-羟甲基胞嘧啶（5-hydroxymethylcytosine, 5-hmC）开展全基因组谱分析。共收集四份精液样本：其中两份分别来自中国医科大学附属盛京医院就诊的健康男性与圆头精子症患者，另外两份来自健康志愿者。参照Illumina官方的"Preparing Samples for CHIP sequencing of DNA"样本制备方案，构建5-羟甲基胞嘧啶富集的基因组DNA文库。采用Illumina Hiseq2000平台进行100bp单端测序，以获取5-羟甲基胞嘧啶富集的DNA片段序列。整体实验设计：检测正常、异常及圆头精子症精子基因组中的5-羟甲基胞嘧啶水平。