Mixed population sequencing of ethanol tolerant Escherichia coli

Parallel, independent colonies of Escherichia coli were gradually evolved to high ethanol tolerance. This experiment resulted in several high ethanol tolerant lines. To capture the mutational trajectories, mixed pools were sequenced from intermediate time points before and after the selective sweep resulting in an increase in tolerance from 6% ethanol to 6.5% ethanol.

将平行独立的大肠杆菌（Escherichia coli）菌落逐步演化，使其获得高乙醇耐受性。本实验最终获得多株高乙醇耐受株系。为捕捉其突变轨迹，研究人员对引发乙醇耐受浓度从6%提升至6.5%的选择性清除事件的前后中间时间点的混合样本池开展了测序。