Expression profiling with RNA-seq between mTORC1 high and low AML cells

We reported the expression profiling between mTORC1 high and low mouse AML cells with MLL-AF9 utilizing a novel fluorescent probe named mVenus-TOSI (TOr-Signal-Indicator). For this purpose, 3 mTOR high (mVenus low) and 4 mTOR low (mVenus high) Mouse AML cells were harvested from 4 mice. The transcriptomic profiles were distinctive and gene ontology analysis revealed that mTORC1 activity was associated with differentially expressed immune response and metabolism related genes. Further, gene signature enrichment analysis (GSEA) validated that the mTORC1 signal signature correlated with mTORC1 activity and that leukemia stem cell (LSC), Myc, and cell cycle related signatures were enriched in mTORC1 high cells.

本研究针对携带MLL-AF9融合基因的小鼠急性髓系白血病（Acute Myeloid Leukemia, AML）细胞，采用一种名为mVenus-TOSI（TOr-Signal-Indicator，靶蛋白信号指示器）的新型荧光探针，开展了高、低哺乳动物雷帕霉素靶蛋白复合物1 (mTORC1) 活性组间的表达谱分析。为此，本研究从4只小鼠体内采集了3株mTORC1高活性（mVenus低表达）与4株mTORC1低活性（mVenus高表达）的小鼠AML细胞。该研究的转录组谱具有显著差异，基因本体（Gene Ontology, GO）分析显示，mTORC1活性与差异表达的免疫应答及代谢相关基因密切相关。进一步的基因集富集分析（Gene Set Enrichment Analysis, GSEA）验证表明，mTORC1信号特征与mTORC1活性显著相关，且白血病干细胞（Leukemia Stem Cell, LSC）、Myc及细胞周期相关特征在mTORC1高活性细胞中显著富集。