Transcriptional changes in lymphoma cells induced by LSD1 depletion

Using RNA-seq we identified the gene expression changes in lymhpoma cells (OCI-Ly1) expressing LSD1 targeted shRNA and control shRNA RNA-seq was performed in biological triplicates from cells selected with puromycin to express non-targeted pLKO1 shRNA against LSD1 (TRCN0000046068 or TCRN0000046072) and cotnrol non-targeted shRNA

本研究通过RNA测序（RNA-seq），鉴定了表达靶向赖氨酸特异性去甲基化酶1 (LSD1) 的短发夹RNA (shRNA) 与对照shRNA的淋巴瘤细胞（OCI-Ly1）中的基因表达差异。本次RNA测序采用三次生物学重复，所用细胞为经嘌呤霉素筛选的稳定细胞株，分别表达克隆于pLKO1载体的靶向LSD1 shRNA（序列编号为TRCN0000046068或TRCN0000046072）以及非靶向对照shRNA。