RNA-seq analysis of LN HIVpos GC-Tfh cells after co-culture with ADA-1 supplementation.

We analyzed with RNA-seq GC-Tfh cells treated in co-culture with SEB, in presence or absence of ADA-1. We used Gene Set Variation Analysis (GSVA) by examining Molecular Signatures Databases (MSigDB) Canonical Pathways. We generated heatmaps illustrating the differential gene expression in the IL-6, IL-2 and Th1/Th2 pathways in HIVpos GC-Tfh cells treated in co-culture with ADA-1 versus SEB alone (Fig 8). This Table shows all the upregulated and downregulated DEGs listed in the IL-6 (first sheet), IL-2 (second sheet) and Th1/Th2 (third sheet) pathways, along with their positive or negative log2-fold change (logFC) as well as nominal p-values. Statistical significance was considered with p (XLSX)

本研究通过RNA测序（RNA-seq）分析了经葡萄球菌肠毒素B（Staphylococcal Enterotoxin B, SEB）刺激，并分别设置添加ADA-1（腺苷脱氨酶1, Adenosine Deaminase 1）与不添加ADA-1的共培养体系中的GC-Tfh细胞（生发中心滤泡辅助T细胞，germinal center follicular helper T cells）。本研究采用基因集变异分析（Gene Set Variation Analysis, GSVA），基于分子特征数据库（Molecular Signatures Database, MSigDB）的经典通路开展分析。本研究绘制了热图，展示了HIV阳性GC-Tfh细胞经ADA-1联合SEB共培养处理，与仅经SEB处理的两组样本中IL-6、IL-2及Th1/Th2通路的差异基因表达情况（图8）。本附表列出了IL-6通路（第1工作表）、IL-2通路（第2工作表）及Th1/Th2通路（第3工作表）中的全部上调与下调差异表达基因（Differentially Expressed Genes, DEGs），并附带其log2倍变化（log2-fold change, logFC）的正负值以及名义p值。本研究以p值作为统计学显著性判定依据，相关文件格式为XLSX。