Combined Effects of Multiple Stresses on Pancreatic Human Islet β-Cell Response: New Insights into the Pathways Involved

This study used multiple common chemical stresses in human islets, mimicking the conditions experienced by dysfunctional β-cells. Proteomic alterations upon exposure of human islets to these stressors were profiled with TMT-based quantitative proteomics and further verified using label-free quantitative proteomics. The differentially expressed proteins under stress conditions were selected for in-depth bioinformatic analysis.

本研究针对人类胰岛施加多种常见化学应激因素，以模拟功能异常β细胞所经历的应激环境。通过基于串联质量标签（TMT）的定量蛋白质组学技术，对人类胰岛暴露于这些应激因素后的蛋白质组变化进行了表征，并采用无标记定量蛋白质组学技术完成了验证。研究筛选出应激条件下的差异表达蛋白，进而开展了深入的生物信息学分析。