A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins, Guichard, Lu, Kanca et al.

Table S1. List of the UAS-SARS-CoV-2 transgenic flies. The docking site and BDSC stock number of each fly line are provided. UAS-NSP lines including a KOZAK sequence to increase expression levels do not include an immunological tag.
Table S2. Available UAS-human cDNA stocks encoding SARS-CoV-2 protein interactors. Stock numbers for Bloomington and Kyoto Drosophila stock centers are indicated. To obtain stocks from Kyoto, an account must be opened at the KDSC (https://kyotofly.kit.jp/cgi-bin/stocks/index.cgi). After placing an order, a letter of No Permit Required (LONPR) must be provided, which can be obtained by requesting at USDA ([e-mail]) and attaching a filled application, which can be found at: https://bdsc.indiana.edu/pdf/USDA/LONPR_form.pdf. For lines that are not available yet, UAS clones are or will soon be available through the DGRC (searchable through https://dgrc.bio.indiana.edu/clones/Catalog under “Human ORF”). Current available clones can be found in https://dgrc.bio.indiana.edu/include/file/DGRC-HumanORF-clone-list-2023-05-05.xlsx.
Table S3. Available T2A-GAL4 or Kozak-GAL4 for fly ortholog candidates that encode for SARS-CoV-2 human interactors. For lines that have not been assigned a BDSC stock number at the time of publication, the CRIMIC number can be entered in the BDSC search bar to locate the stock number and line as it becomes available.
Table S4. Summary of lethality phenotypes induced by SARS-CoV-2 factors driven by DaGAL4 at 29oC, indicating the developmental timing of lethality.
Table S5. List of human interactors identified in five interactome studies 9 7,11 12 10, showing which interactors have been identified again in subsequent studies. Candidates identified in the 9 study are outlined in yellow.

表S1. UAS-SARS-CoV-2转基因果蝇列表。本列表提供了每个果蝇品系的停靠位点以及布卢明顿果蝇种质中心（Bloomington Drosophila Stock Center, BDSC）的品系编号。带有科扎克（KOZAK）序列以提升表达水平的UAS-NSP系列品系未携带免疫标签。

表S2. 可获取的编码SARS-CoV-2蛋白互作因子的UAS-人cDNA种质资源。本列表标注了布卢明顿果蝇种质中心（BDSC）与京都果蝇种质中心（Kyoto Drosophila Stock Center, KDSC）的品系编号。若需获取京都果蝇种质中心的品系，需先在KDSC官网（https://kyotofly.kit.jp/cgi-bin/stocks/index.cgi）注册账号。下单后需提供无许可证明函（Letter of No Permit Required, LONPR）：可通过发送申请至美国农业部（USDA）邮箱[e-mail]，并填写附件申请表获取，申请表下载链接为：https://bdsc.indiana.edu/pdf/USDA/LONPR_form.pdf。对于暂未公开的品系，其UAS克隆可通过果蝇基因组资源中心（Drosophila Genomics Resource Center, DGRC）获取（可通过https://dgrc.bio.indiana.edu/clones/Catalog 中的"Human ORF"栏目检索）。当前可获取的克隆列表可访问：https://dgrc.bio.indiana.edu/include/file/DGRC-HumanORF-clone-list-2023-05-05.xlsx。

表S3. 可获取的T2A-GAL4或Kozak-GAL4品系，对应编码SARS-CoV-2人类互作因子的果蝇同源候选基因。若在文章发表时尚未分配BDSC品系编号，可在BDSC检索栏中输入CRIMIC编号，待品系上线后即可获取对应的品系信息。

表S4. 29℃条件下由DaGAL4驱动的SARS-CoV-2因子诱导的致死表型汇总，表格标注了致死发生的发育时期。

表S5. 五项互作组研究[9, 7, 11, 12, 10]中鉴定出的人类互作因子列表，展示了后续研究中再次鉴定到的互作因子。其中9号研究鉴定的候选因子以黄色标注。