Table 1_Cluster analysis allowed to identify antifungal drugs that retain efficacy against Candida albicans isolated from patients with inflammatory diseases of the soft tissues of the maxillofacial area.docx

Physicians are increasingly prescribing antifungal drugs empirically to treat hospital-acquired infections quickly. This makes it obvious that fungal infections require more attention and systematic monitoring of resistance among them. The aim of the study was to identify antifungal drugs that retain their efficacy against C. albicans isolates. There were 17 clinical isolates of Candida albicans obtained from patients and tested for susceptibility to antifungal drugs using the standard double dilution method. Amphotericin B, fluconazole, itraconazole, micafungin, and posaconazole were used in the study. To determine the groups of antimycotics to which the studied microorganisms retain sensitivity, a hierarchical cluster analysis was performed using the Ward's method. The tested representatives of the genus Candida showed the lowest sensitivity to fluconazole. The efficacy of amphotericin B and itraconazole was almost at the same level. In turn, micafungin and posaconazole showed the best results against C. albicans isolates. Ward's cluster analysis combined the results of C. albicans susceptibility to fluconazole, micafungin and itraconazole by the highest mathematical similarity. Amphotericin B and posaconazole were combined into one cluster due to their better efficacy against Candida albicans isolates.

临床医师愈发倾向于经验性开具抗真菌药物，以快速治疗医院获得性感染。这凸显出真菌感染者的耐药性亟需得到更多关注与系统化监测。本研究的目标是筛选出对白色念珠菌（Candida albicans）分离株仍保持抗菌活性的抗真菌药物。本研究从患者体内分离得到17株临床白色念珠菌，并采用标准两倍稀释法检测其对抗真菌药物的敏感性。本研究选用的受试药物包括两性霉素B（Amphotericin B）、氟康唑（fluconazole）、伊曲康唑（itraconazole）、米卡芬净（micafungin）与泊沙康唑（posaconazole）。为明确受试菌株对哪些抗真菌药物类别保持敏感性，本研究采用沃德法（Ward's method）进行系统聚类分析。受试的念珠菌属菌株对氟康唑的敏感性最低。两性霉素B与伊曲康唑的抗菌活性水平基本相当。相较而言，米卡芬净与泊沙康唑对白色念珠菌分离株展现出最优的抗菌效果。沃德聚类分析基于最高的数学相似度，将白色念珠菌对氟康唑、米卡芬净与伊曲康唑的敏感性结果归为一类。两性霉素B与泊沙康唑则因对白色念珠菌分离株具有更优异的抗菌活性，被归为同一聚类簇。