lncRNA profile of PANC-1 treated with CAFs derived EVs. lncRNA profile of PANC-1 treated with CAFs derived EVs

Perineural invasion (PNI) is a unique biological feature of pancreatic cancer and is a key cause of pancreatic cancer metastasis, recurrence and poor postoperative survival, but its mechanism is largely unclarified. Clinical sample analysis and endoscopic ultrasonographic elasticity scoring indicated that cancer-associated fibroblasts (CAFs) are closely related to the occurrence of PNI. Furthermore, CAF-derived extracellular vesicles played an extremely important role in PNI in a dorsal root ganglion (DRG) coculture model and sciatic nerve model. To explore the lncRNA packaged in CAFs EVs transmitted to PANC-1, we conducted the lncRNA profile of PAN-1 treated with CAFs derived EVs. Overall design: CAFs were isolated from pancreatic cancer and conditional medium were collected for EVs isolation. PANC-1 were treated with CAF-derived EVs and then the RNA were extracted for lncRNA-seq.

神经周围侵犯（Perineural invasion, PNI）是胰腺癌特有的生物学特征，亦是胰腺癌转移、复发及术后预后不良的关键诱因，但其具体机制目前仍未被充分阐明。临床样本分析与内镜超声弹性评分结果表明，癌相关成纤维细胞（cancer-associated fibroblasts, CAFs）与PNI的发生密切相关。进一步研究证实，在背根神经节（dorsal root ganglion, DRG）共培养模型及坐骨神经模型中，癌相关成纤维细胞来源的细胞外囊泡（extracellular vesicles, EVs）在PNI进程中发挥了至关重要的作用。为探究癌相关成纤维细胞来源细胞外囊泡所包裹并传递至PANC-1细胞的长链非编码RNA（long non-coding RNA, lncRNA），我们对经该类细胞外囊泡处理后的PANC-1细胞开展了长链非编码RNA表达谱分析。整体实验设计：本研究从胰腺癌组织中分离癌相关成纤维细胞，收集其条件培养基以提取细胞外囊泡；将PANC-1细胞经癌相关成纤维细胞来源的细胞外囊泡处理后，提取总RNA并进行长链非编码RNA测序（lncRNA-seq）。