Effect of Na2S on partenogenetic development of porcine oocytes.
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Oocytes were matured with or without Na2S and partenogenetically activated using calcium ionophore. Pronucleus formation after 24 h zygote culture, cleavage rate after 2 days and blastocyst achievement after 7 days presumptive embryos culture were evaluated (%±SE).H2S: 300 µM Na2S during oocyte maturation.*Statistically significant differences between control and H2S group – in column (P
本研究将卵母细胞分为两组，分别在添加或不添加硫化钠（Na₂S）的培养液中进行成熟培养，随后采用钙离子载体（calcium ionophore）对两组卵母细胞实施孤雌激活。分别检测各组受精卵培养24小时后的原核形成率、培养2天后的卵裂率，以及推定胚胎培养7天后的囊胚形成率，检测结果以平均值±标准误（% ± SE）表示。硫化氢（H₂S）处理组为在卵母细胞成熟阶段添加300 µM硫化钠（Na₂S）。*对照组与硫化氢组间差异具有统计学意义（列中，P
创建时间：
2015-12-02
